Translational_Unit
gpS

Part:BBa_K836006

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-20)


Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

This gene codifies for a protein that makes holes in the inner membrane of the bacterial cell. A Gram positive RBS (SpoVG, https://parts.igem.org/wiki/index.php?title=Part:BBa_K143021) was added at the beginning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 275
    Illegal BsaI.rc site found at 188


[edit]
Categories
//cds/lysis
//cds/membrane/lysis
//function/celldeath
Parameters
None